pkh26 fluorescent dye Search Results


red fluorescent membrane dye pkh26  (PTI Research Inc)
90
PTI Research Inc red fluorescent membrane dye pkh26
Red Fluorescent Membrane Dye Pkh26, supplied by PTI Research Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh26+fluorescent+dye/red+fluorescent+membrane+dye+pkh26/pmc06360510__Data_Sheet_1-23-6-11
Average 90 stars, based on 1 article reviews
red fluorescent membrane dye pkh26 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
pkh26 fluorescent dye  (Yeasen Biotechnology)
86
Yeasen Biotechnology pkh26 fluorescent dye
Pancreatic cancer‐derived EVs induce skeletal muscle wasting. A) Left: Experimental scheme: normal pancreas tissues of nonPDAC patients and pancreatic tumor tissues of PDAC patients were collected in sterile procedures and cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. B) The quantitative analysis of myotube diameters of C2C12 cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells (n = 3). C) Western blot of MHC protein in C2C12 myoblasts cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells. D) Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were injected to mice intravenously for 2 weeks. E—G) Grip strength changes (E), GA muscle images (F), and muscle weight analysis (G) of mice injected with EVs from plasma of control patients (Con, n = 6) and PDAC patients (PDAC, n = 6). H) HE staining of the GA muscle from mice injected with EVs from the plasma of control patients and PDAC patients. Scale bars, 100 µm. I) Quantification of the myofiber CSA in Figure (n = 6). J) Left: Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. K) The quantitative analysis of myotube diameters of C2C12 treated with EVs extracted from patients, mice, organoids, and Pan02 cells (n = 3). L) Western blot of MHC protein in C2C12 myoblasts cocultured with EVs of patients, mice, organoids, and Pan02 cells. M) Fluorescence imaging of mouse muscle, liver, bone, and brain tissues after mice were injected with <t>PKH26‐stained</t> EVs from Pan02 cells. N) Experimental scheme: Pan02 mice were injected with AAV‐palmmCherry directly into their tumors. O) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐tumor‐injected mice. Scale bars, 100 µm. P) Dot‐plots of EVs from mice injected with AAV‐palmmCherry in their tumors and EVs from mice not injected with AAV‐palmmCherry in their tumors using nano‐flow cytometry. Q) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐EVs‐injected mice. Scale bars, 50 µm. R) Experimental scheme: Mice were injected with PKH26‐labeled EVs extracted from tumor‐bearing mice. S) Representative immunofluorescent images of PKH26 and Laminin in the muscle of PKH26‐labeled‐EVs‐injected mice. Scale bars, 50 µm. Data shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
Pkh26 Fluorescent Dye, supplied by Yeasen Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh26+fluorescent+dye/dye+fluorescent+pkh26/pmc12499426-309-6-9
Average 86 stars, based on 1 article reviews
pkh26 fluorescent dye - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
pkh26 fluorescent dye  (REGEN Biotech Inc)
90
REGEN Biotech Inc pkh26 fluorescent dye
Pancreatic cancer‐derived EVs induce skeletal muscle wasting. A) Left: Experimental scheme: normal pancreas tissues of nonPDAC patients and pancreatic tumor tissues of PDAC patients were collected in sterile procedures and cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. B) The quantitative analysis of myotube diameters of C2C12 cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells (n = 3). C) Western blot of MHC protein in C2C12 myoblasts cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells. D) Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were injected to mice intravenously for 2 weeks. E—G) Grip strength changes (E), GA muscle images (F), and muscle weight analysis (G) of mice injected with EVs from plasma of control patients (Con, n = 6) and PDAC patients (PDAC, n = 6). H) HE staining of the GA muscle from mice injected with EVs from the plasma of control patients and PDAC patients. Scale bars, 100 µm. I) Quantification of the myofiber CSA in Figure (n = 6). J) Left: Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. K) The quantitative analysis of myotube diameters of C2C12 treated with EVs extracted from patients, mice, organoids, and Pan02 cells (n = 3). L) Western blot of MHC protein in C2C12 myoblasts cocultured with EVs of patients, mice, organoids, and Pan02 cells. M) Fluorescence imaging of mouse muscle, liver, bone, and brain tissues after mice were injected with <t>PKH26‐stained</t> EVs from Pan02 cells. N) Experimental scheme: Pan02 mice were injected with AAV‐palmmCherry directly into their tumors. O) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐tumor‐injected mice. Scale bars, 100 µm. P) Dot‐plots of EVs from mice injected with AAV‐palmmCherry in their tumors and EVs from mice not injected with AAV‐palmmCherry in their tumors using nano‐flow cytometry. Q) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐EVs‐injected mice. Scale bars, 50 µm. R) Experimental scheme: Mice were injected with PKH26‐labeled EVs extracted from tumor‐bearing mice. S) Representative immunofluorescent images of PKH26 and Laminin in the muscle of PKH26‐labeled‐EVs‐injected mice. Scale bars, 50 µm. Data shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.
Pkh26 Fluorescent Dye, supplied by REGEN Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pkh26+fluorescent+dye/pkh26+fluorescent+dye/pm24909763-59-3-14
Average 90 stars, based on 1 article reviews
pkh26 fluorescent dye - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


Pancreatic cancer‐derived EVs induce skeletal muscle wasting. A) Left: Experimental scheme: normal pancreas tissues of nonPDAC patients and pancreatic tumor tissues of PDAC patients were collected in sterile procedures and cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. B) The quantitative analysis of myotube diameters of C2C12 cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells (n = 3). C) Western blot of MHC protein in C2C12 myoblasts cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells. D) Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were injected to mice intravenously for 2 weeks. E—G) Grip strength changes (E), GA muscle images (F), and muscle weight analysis (G) of mice injected with EVs from plasma of control patients (Con, n = 6) and PDAC patients (PDAC, n = 6). H) HE staining of the GA muscle from mice injected with EVs from the plasma of control patients and PDAC patients. Scale bars, 100 µm. I) Quantification of the myofiber CSA in Figure (n = 6). J) Left: Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. K) The quantitative analysis of myotube diameters of C2C12 treated with EVs extracted from patients, mice, organoids, and Pan02 cells (n = 3). L) Western blot of MHC protein in C2C12 myoblasts cocultured with EVs of patients, mice, organoids, and Pan02 cells. M) Fluorescence imaging of mouse muscle, liver, bone, and brain tissues after mice were injected with PKH26‐stained EVs from Pan02 cells. N) Experimental scheme: Pan02 mice were injected with AAV‐palmmCherry directly into their tumors. O) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐tumor‐injected mice. Scale bars, 100 µm. P) Dot‐plots of EVs from mice injected with AAV‐palmmCherry in their tumors and EVs from mice not injected with AAV‐palmmCherry in their tumors using nano‐flow cytometry. Q) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐EVs‐injected mice. Scale bars, 50 µm. R) Experimental scheme: Mice were injected with PKH26‐labeled EVs extracted from tumor‐bearing mice. S) Representative immunofluorescent images of PKH26 and Laminin in the muscle of PKH26‐labeled‐EVs‐injected mice. Scale bars, 50 µm. Data shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Advanced Science

Article Title: Pancreatic Cancer‐Derived Extracellular Vesicles Enriched with miR‐223‐5p Promote Skeletal Muscle Wasting Associated with Cachexia

doi: 10.1002/advs.202504064

Figure Lengend Snippet: Pancreatic cancer‐derived EVs induce skeletal muscle wasting. A) Left: Experimental scheme: normal pancreas tissues of nonPDAC patients and pancreatic tumor tissues of PDAC patients were collected in sterile procedures and cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. B) The quantitative analysis of myotube diameters of C2C12 cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells (n = 3). C) Western blot of MHC protein in C2C12 myoblasts cocultured with pancreas tissues of patients and mice, and CM of organoids and Pan02 cells. D) Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were injected to mice intravenously for 2 weeks. E—G) Grip strength changes (E), GA muscle images (F), and muscle weight analysis (G) of mice injected with EVs from plasma of control patients (Con, n = 6) and PDAC patients (PDAC, n = 6). H) HE staining of the GA muscle from mice injected with EVs from the plasma of control patients and PDAC patients. Scale bars, 100 µm. I) Quantification of the myofiber CSA in Figure (n = 6). J) Left: Experimental scheme: EVs isolated from the plasma of patients with PDAC (PDAC‐EVs) and healthy individuals (Con‐EVs) were cocultured with C2C12 myoblasts. Right: The representative images of immunofluorescence staining for MHC in C2C12 myoblasts. Scale bars, 100 µm. K) The quantitative analysis of myotube diameters of C2C12 treated with EVs extracted from patients, mice, organoids, and Pan02 cells (n = 3). L) Western blot of MHC protein in C2C12 myoblasts cocultured with EVs of patients, mice, organoids, and Pan02 cells. M) Fluorescence imaging of mouse muscle, liver, bone, and brain tissues after mice were injected with PKH26‐stained EVs from Pan02 cells. N) Experimental scheme: Pan02 mice were injected with AAV‐palmmCherry directly into their tumors. O) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐tumor‐injected mice. Scale bars, 100 µm. P) Dot‐plots of EVs from mice injected with AAV‐palmmCherry in their tumors and EVs from mice not injected with AAV‐palmmCherry in their tumors using nano‐flow cytometry. Q) Representative immunofluorescent images of mCherry and Laminin in the muscle of AAV‐palmmCherry‐EVs‐injected mice. Scale bars, 50 µm. R) Experimental scheme: Mice were injected with PKH26‐labeled EVs extracted from tumor‐bearing mice. S) Representative immunofluorescent images of PKH26 and Laminin in the muscle of PKH26‐labeled‐EVs‐injected mice. Scale bars, 50 µm. Data shown as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: To effectively monitor EVs trafficking, the PKH26 fluorescent dye (Yeasen, China) was utilized to label EVs.

Techniques: Derivative Assay, Sterility, Immunofluorescence, Staining, Western Blot, Isolation, Clinical Proteomics, Injection, Control, Fluorescence, Imaging, Flow Cytometry, Labeling